P7306
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P7306
Sigma
Polyethylene glycol solution
Hybri-Max™, 50 % (w/v) in 10% aqueous DMSO (v/v), average mol wt 1,450, sterile-filtered, hybridoma tested
| Synonym: | PEG solution |
| CAS Number: | 25322-68-3 |
| MDL number: | MFCD00081839 |
| PubChem Substance ID: | 24898839 |
Description
| Application | Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO. |
| PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization. | |
| Other Notes | Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium. |
| Packaging | Packaged in sealed ampules under nitrogen. |
| Reconstitution | Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles. |
| Legal Information | Hybri-Max is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| grade | Hybri-Max™ |
| sterility | sterile-filtered |
| mol wt | average mol wt 1,450 |
| concentration | 50 % (w/v) in 10% aqueous DMSO (v/v) |
| total impurities | Endotoxin, tested |
| suitability | hybridoma tested |
| storage temp. | 2-8°C |
Safety
| WGK Germany | 1 |
References
| reference | , Harlow, E., and Lane, D., ed. Antibodies: A Laboratory Manual Cold Spring Harbor, NY , (1998), 211-213 |
| Davidson, R.L., and Gerald, P.S., Induction of mammalian somatic cell hybridization by polyethylene glycol. Methods Cell Biol. 15, 325-338, (1977) | |
| Merck | Merck 13,7651 |
